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«For research use only E and LE-Series USER MANUAL an automated, batch system for high performance sample preparation using adaptive focused acoustic ...»

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For research use only

E and LE-Series

USER MANUAL

an automated, batch system for

high performance sample preparation

using adaptive focused acoustic energy

Product Name: E/LE-series running SonoLab 6.2.4 or later

Product Number: E220, E220x, LE220, E220R, LE220R

E/LE-series User Manual 1

UNIVERSAL PRECAUTIONS

Universal Precautions should be followed on all specimen samples, regardless of whether a sample is known to contain an infectious agent. Laboratories handling specimen samples are advised to comply with applicable parts of the following

governmental and clinical standards, or their equivalent in the country of use:

• Centers for Disease Control (CDC), Universal Precautions for Prevention of Transmission of HIV and Other Bloodborne Infections, published 1987, updated 1996

• Clinical and Laboratory Standards Institute (CLSI), GP17-A2 Clinical Laboratory Safety; Approved Guideline Second Edition, published 2004, ISBN 1-56238-530-5

• Clinical and Laboratory Standards Institute (CLSI), M29-A3 Protection of Laboratory Workers from Occupationally Acquired Infections; Approved Guideline, Third Edition, published 2005, ISBN 1-56238-5674

• Occupational Safety and Health Administration (OSHA), 29 CFR 1910.1030 Bloodborne Pathogens

• International Standards Organization (ISO) 15190:2003, Medical Laboratories – Requirements for Safety Trademarks Patented or patent pending and registered or registration-pending trademarks of Covaris are protected.

Registered names and trademarks used in this document, even when not specifically marked as such, are not to be considered unprotected by law.

Information subject to change without notice For Research Use Only Not for use in diagnostic procedures Publication P/N 010119 Rev I March 2016 Covaris products are covered by Patent No. US 6,719,449, US 6,948,843, and other pending applications Copyright ©2014 Covaris, all rights reserved.

Covaris, Inc.

14 Gill Street, Unit H Woburn, Massachusetts 01801-1721 USA Printed in U.S.A.

E/LE-series User Manual 2 Warnings

For safety of operating personnel:

1. Make sure that the equipment is properly grounded. DO NOT operate if it is not properly grounded.

2. The unit is equipped with a power plug appropriate for the destination country. DO NOT, under any circumstances, remove the grounding prong from the power cord.

3. DO NOT attempt to operate the equipment without the safety door closed. When the safety door is open, the mechanical positioning system and the acoustic wave system will not run. If there is any indication that the safety system is not functioning properly, DO NOT operate the equipment and contact Covaris immediately.

4. If the equipment is used in a manner not specified by the manufacturer, the protection provided by the equipment may be impaired.

To prevent damage to the equipment:

1. The instrument is designed to operate in ambient laboratory conditions e.g., 15 to 32°C (60 to 90°F). DO NOT operate the instrument in a cold room environment; the system is designed to operate with a water bath and re-circulating heater/chiller apparatus to control sample temperatures.

2. NEVER run a method without a water bath; this could damage the transducer. The instrument is equipped with a water level sensor to protect the transducer. The system will not allow the acoustic wave treatment to start unless an adequate volume of water is present.

3. ONLY distilled or deionized water should be used to fill the water bath.

4. Empty the water bath and wipe dry EVERY day. DO NOT leave water in the tank for extended time as there is no water filtration or water cleaning system with the apparatus. Drain the degassing lines (see section 4.5 Shutdown Procedure).

5. Establish a standard of operation and periodically test equipment, as described in the Maintenance section of this manual.

6. Be advised that loading third party software onto the computer may interfere with the performance of the Covaris SonoLab™ software. Please consult Covaris.

E/LE-series User Manual 3 Warranty When used in accordance with written instruction and under normal operating conditions, the Covaris instruments are guaranteed to be free of defects in MATERIAL and WORKMANSHIP for one (1) year from the date of original delivery. Any component which proves defective during the stated period will be repaired free of charge or replaced at the sole discretion of Covaris, F.O.B., Woburn, Massachusetts, provided the defective component is returned properly packaged with all transportation charges prepaid. The customer is expected to perform basic diagnostics and component replacement with telephone support from Covaris personnel. If Covaris personnel are required to perform on-site repair, all travel costs are paid by the customer. A limited warranty as specified may apply to certain components of the equipment.

Warranty Exceptions This warranty is void if failure of the software or hardware has resulted from accidents, abuse, improper maintenance, or repair, or misapplication by the customer. It is also void if damage is caused by any unauthorized attachments or if modifications are made to the equipment. Removing or tampering with the Safety Enclosure will void the warranty, and the customer will assume all liabilities.





This warranty is limited to the original purchaser and is not transferable.

The software will perform according to the accompanying written materials and the medium on which the software is delivered is free of defects in materials under normal use and service. The warranty is void if damage has resulted from third party software not intended for use with the system.

The high power focused transducer is designed to give maximal mechanical energy output in water.

Permanent damage to the transducer and electronic circuits could result if the transducer is operated without water. Operation of the system without water in the water bath voids the warranty.

CONTACT COVARIS, INC. SHOULD YOU HAVE ANY QUESTIONS CONCERNING EQUIPMENT.

Warranty Services The purchased equipment is covered by a twelve (12) month warranty which includes all the service and support necessary so that the customer can operate the equipment successfully. Extended warranties are available at the end of the original 12 month warranty period.

Services included with the original purchase of the system are:

Installation and Training – Setup and installation of the equipment and operator training can be purchased at time of initial acquisition of the equipment. Both will be performed by a qualified Covaris service representative. One half day will be scheduled to perform the installation and on-site training.

• The operators’ training will include valuable hands-on time with the equipment.

• Preventative maintenance and troubleshooting tips will also be covered.

Technical Support – On-going assistance with the operation or application of the equipment and/or

troubleshooting is provided via:

• Telephone (+1 781 932 3959) during the hours of 9:00am to 4:00pm, Monday through Friday, United States, Eastern Standard Time (EST).

o Greenwich Mean Time (GMT-05:00) o

• E-mail queries to techsupport@covarisinc.com Parts Replacement – Replacement of parts (excluding consumables and cables) for normal operation of equipment are provided on a priority basis. Shipping charges are included. Failure due to accident, abuse, or improper operation is not covered.

E/LE-series User Manual 4

TABLE OF CONTENTS

1.0 INTRODUCTION

1.1 Overview of the Manual

1.2 Purpose of the Equipment

1.3 Main Components

1.4 Principle of Operation

1.5 System Distinctions – E, Ex and LE

2.0 INSTALLATION

2.1 Unpacking and System Setup

2.2 Placement of Equipment

2.3 Power Requirements

2.4 Set-up Procedures

2.4.1 Connections

2.4.2 Powering Up the System

2.4.3 Control Buttons

2.4.4 Water Bath Configuration

3.0 SYSTEM DESCRIPTION

3.1 General Overview

3.2 Treatment System

3.2.1 Acoustic Transducer

3.2.2 Temperature Sensors and Temperature Control

3.2.3 Sample Positioning System

3.2.4 Water Bath and Degassing System

3.3 Computer

3.4 Safety System

3.5 Water Bath Temperature

3.6 Application Software

3.6.1 Main Panel

3.6.2 Method Editor Panel

4.0 SYSTEM OPERATION

4.1 Start-Up Procedures

4.2 Creating and Modifying a Method in the Method Editor Panel

4.3 Running a Method Manually in the Main Panel

4.4 Manual Overrides

4.5 Shut-Down Procedures

5.0 SYSTEM SPECIFICATIONS

6.0 MAINTENANCE

6.1 Water Bath

6.1.1 Recommendations for Daily Maintenance

6.1.2 Recommendations for Monthly Maintenance

6.2 Transducer

6.3 Safety System

6.4 Degassing the System

6.5 Relocation of the System

6.5.1 Prepare the System

6.5.2 Move of the System

6.5.3 Reinstall the System

7.0 TROUBLESHOOTING

APPENDIX A: Sonolab 7 And 6.2 Software Installation on a Windows XP Or 7 Computer................ 26 APPENDIX B: Covaris 220 Series Instrument Driver Update Procedure for Windows 7

APPENDIX C: Site Preparation and Equipment Installation Requirements

APPENDIX D: Humidity and Water Management in Covaris E and LE Systems

APPENDIX E: Recommended E220(X) or LE220 IQ / OQ / PQ

APPENDIX F: “R” version of E220 and LE220 (E220R and LE220R)

–  –  –

There is also a PDF version of this manual on the system computer (if a computer was supplied with your order). Open the manual by double clicking the manual icon on the desktop.

1.2 Purpose of the Equipment The E and LE Series instruments are used for high-throughput, sample preparation and extraction. The technology uses controlled and highly focused nonlinear acoustic waves that are delivered to each sample. The systems are designed to work with a variety of closed or open sample vessels including vials, tubes, and multi-well plates. Contact Technical Support for more information. The instruments are not intended for use in diagnostic procedures; for research use only.

–  –  –

1.4 Principle of Operation The Covaris Process generates acoustic shock waves to cause controlled cavitation and acoustic streaming at the focal point within the sample treatment vessel. Acoustic energy has been utilized for many years for a variety of diagnostic, therapeutic and research purposes. Wellknown uses of sonic or acoustic energy in materials processing include "sonication", an uncontrolled process of mechanical disruption involving the direct immersion of an unfocused kHz range (e.g., 15,000 cycles per second) ultrasound source into a fluid suspension of the material being treated. There are also specific clinical examples of the utilization of therapeutic ultrasound (e.g. lithotripsy) and of diagnostic ultrasound (e.g., fetal imaging).

Typically, when ultrasound is applied to a bulk biological sample solution (as for the extraction of intracellular constituents from tissue), the treatment causes a complex, heterogeneous, mixture of sub-events that vary during the course of a treatment dose. In other words, the ultrasonic energy may be partitioned between various states. For example, the energy may directly treat a sample;

the energy may displace the target and shift the target out of the optimal energy zone; the energy may result in interference, (i.e., a “bubble shield”), that reflects the acoustic energy; or the large particles may move to low energy nodes thereby leaving the smaller particles with more dwelltime in the high energy nodes. In addition, the sample viscosity, temperature, and uniformity will vary during the ultrasonic process, resulting in gradients during the process. The current low frequency, unfocused sonicator-type processes are random and non-uniform. This limits the use of ultrasound in high throughput applications where treatment standardization is required.

In contrast, focused, nonlinear acoustic waves are computer generated and delivered to individually-addressable samples with the E and LE series systems. The mechanical energy imparted on the sample results in a controlled series of compression, rarefaction events. The E and LE-series instruments were designed to provide the researcher with methods and systems to control the delivery of mechanical energy into a biological sample for high-throughput sample preparation applications.

–  –  –

Graphics employed in this manual will show the LE well plate configuration, but all instructions are applicable to either E, Ex, or LE systems.

E/LE-series User Manual 8

2.0 INSTALLATION 2.1 Unpacking and System Setup The Covaris system is shipped in one crate, containing the instrument, the instrument supplies, optionally purchased notebook computer, and the selected accessory kit. Remove all wrappings, taking care not to inadvertently discard any parts, accessories, or documents.

Normal precautions in unpacking and reasonable care in handling should be exercised to avoid possible damage to the unit. Visually inspect all external controls, indicators and surfaces to detect any damage that may have occurred during shipment.

The shipping company is responsible for damage to equipment during shipment. If damage has occurred, notify the shipping company immediately to establish proper basis for a claim.

NOTE: In case of damage do not discard packing material until Covaris has inspected for cause of damage.

WARNING: Risk of back injury. The instrument weighs approximately 110 pounds (50 kilograms).



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